It enters the exonuclease active site, where the mismatched bond is hydrolytically cleaved. The coordination ensures that the replication fork advances smoothly as the enzyme continuously adds nucleotides to the leading and lagging strands.
Reactive Chemistry Alignment in DNA Polymerase Nucleotide Addition
Adenine is positioned to pair with thymine, and guanine is positioned to pair with cytosine. These interactions increase the processivity of the enzyme, allowing it to add thousands of nucleotides without dissociating.
The Chemical Mechanism of Bond Formation The actual chemistry involves a nucleophilic attack by the 3' hydroxyl group of the growing chain. The Template-And-Nutrient Principle The core principle governing how DNA polymerase adds nucleotides is strict complementarity.
Reactive Chemistry Alignment in DNA Polymerase Nucleotide Addition
DNA polymerase is not a static clamp; it undergoes conformational changes upon nucleotide binding. This oxygen atom attacks the alpha-phosphate of the incoming dNTP, displacing the beta and gamma phosphates as pyrophosphate.
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