It enters the exonuclease active site, where the mismatched bond is hydrolytically cleaved. Coordination With The Replication Machinery In a living cell, the process does not occur in isolation.
DNA Polymerase Geometry Check Ensures Accurate Nucleotide Addition
Only when the geometry is perfect does the enzyme proceed to the next stage, preventing the incorporation of incorrect bases. This induced fit aligns the reactive chemistry perfectly and excludes incorrect nucleotides that lack the proper geometry.
Understanding this mechanism reveals how life maintains its genetic code across generations. DNA polymerase is not a static clamp; it undergoes conformational changes upon nucleotide binding.
DNA Polymerase Geometry Check Ensures Accurate Nucleotide Addition
DNA polymerase interacts with sliding clamps and other accessory proteins that tether it to the template. The result is the formation of a phosphodiester bond that links the new nucleotide to the chain.
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