The structural rearrangement effectively "locks" the correct base in place before the bond is finalized. DNA polymerase interacts with sliding clamps and other accessory proteins that tether it to the template.
Ensuring Fidelity How DNA Polymerase Prevents Incorrect Base Incorporation
The result is the formation of a phosphodiester bond that links the new nucleotide to the chain. This oxygen atom attacks the alpha-phosphate of the incoming dNTP, displacing the beta and gamma phosphates as pyrophosphate.
Activation Through Triphosphate Bond Hydrolysis Energy for the synthesis reaction is embedded in the structure of the nucleotide itself. Coordination With The Replication Machinery In a living cell, the process does not occur in isolation.
Ensuring Fidelity: How DNA Polymerase Prevents Incorrect Base Incorporation
Only when the geometry is perfect does the enzyme proceed to the next stage, preventing the incorporation of incorrect bases. The coordination ensures that the replication fork advances smoothly as the enzyme continuously adds nucleotides to the leading and lagging strands.
More About How does dna polymerase add nucleotides
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