Operational Procedure: From Sample Application to Enumeration The operational workflow of the petrifilm aerobic count plate is designed for speed and minimal hands-on complexity, reducing the potential for human error. Directly coated onto this base is a thin, uniform layer of gel that serves as the growth matrix.
Petrifilm Aerobic Count Plate Guide: From Sample Application to Enumeration
The film is then peeled back and stacked, or placed in a standard petri dish, and incubated at 35°C ± 1°C for a total duration of 72 hours. 45 µm, which acts as a barrier.
This dehydrated culture medium, housed within a sterile, ready-to-use film format, streamlines the traditionally labor-intensive process of plate counting into an efficient, semi-automated procedure. This gel is composed of a nutrient-rich agar formulation specifically tailored to support the growth of aerobic mesophilic organisms, alongside a critical bottom layer of cold-water-soluble gelling agent.
From Sample Application to Enumeration: Mastering the Petrifilm Aerobic Count Plate Workflow
Crucially, the top layer is a sterile, porous membrane filter with a defined pore size, usually 0. The critical step follows: using the provided spreading rod, the sample is carefully and evenly distributed across the entire counting area.
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