Because the sample is held as a droplet between two optical surfaces, the physical height of the liquid column can vary. Users should ensure the pedestal is clean and dry before placing a sample, and that the droplet is correctly positioned to span the measurement window.
Nanodrop Spectrophotometry Workflow Guide: Step-by-Step Measurement Process
Using pre-programmed extinction coefficients for nucleic acids and proteins, the device calculates concentration and purity ratios, such as the A260/A280 ratio, which indicates the presence of protein contamination. Considerations and Limitations While highly effective, users must be aware of the limitations inherent in the technology.
This ratio of intensities is the transmittance, which the software converts into absorbance units. Proper cleaning of the pedestal between measurements is essential to prevent cross-contamination and ensure consistent performance, particularly when analyzing samples with varying concentrations.
Nanodrop Spectrophotometry Workflow Guide: Step-by-Step Measurement Process
To ensure accuracy, the instrument dynamically measures the path length of the light traveling through the sample. Eliminating the need for disposable cuvettes and extensive sample preparation reduces waste and saves valuable time in the laboratory.
More About How does the nanodrop work
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