Furthermore, the digital read-out ensures high accuracy, while the spatial resolution available on certain platforms reveals the precise location of molecular events within a tissue microenvironment. Neuroscience and Gene Expression Mapping For neuroscientists, unraveling the cellular heterogeneity of the brain requires tools that can distinguish between similar cell types with high fidelity.
Advantages Over QPCR and NGS: Superior Accuracy and Spatial Resolution
nanostring enables the precise measurement of thousands of transcripts in single cells or specific brain layers, revealing the molecular pathways that govern cognition and behavior. These barcoded molecules hybridize directly with the target sequences in fixed cells, tissues, or purified nucleic acids, maintaining the spatial context of the original sample.
This integration into clinical workflows represents a significant step toward truly precision healthcare. In oncology, it is routinely employed to validate targeted therapy candidates, classify cancer subtypes, and predict patient response to treatment through immuno-oncology profiling.
Advantages Over QPCR NGS: Digital Accuracy and Spatial Resolution
The Foundational Technology of Digital Spatial Profiling The ingenuity of nanostring lies in its use of single-molecule detection to eliminate the background noise common in other platforms. A high-resolution digital imager then reads these barcodes, counting each distinct molecule to provide absolute quantification without the need for standard curves.
More About What is nanostring
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